Please use this identifier to cite or link to this item: http://hdl.handle.net/10525/2396

 Title: A statistical test vs. a validation experiment in Gene Expression Study Authors: Noncheva, VeskaDobreva, Maria Keywords: statistical hypothesesone sample location testgene expression Issue Date: 29-May-2014 Publisher: Institute of Mathematics and Informatics Bulgarian Academy of Sciences, Association for the Development of the Information Society Citation: Proceedings of the National Conference on "Education and Research in the Information Society", Plovdiv, May, 2014, 148p-153p Series/Report no.: ADIS;2014 Abstract: Comparative CT Method compares the Ct value of one target gene to another using the formula called 2-ΔΔCT. To make this method valid, the efficiency of the target amplification (the gene of interest) and the efficiency of the reference amplification (the endogenous control) must be equal. In this article we propose to test statistical hypotheses instead to perform validation biological experiments when we want to show that the efficiencies of the target and endogenous control amplifications are approximately equal. Description: Report published in the Proceedings of the National Conference on "Education and Research in the Information Society", Plovdiv, May, 2014 URI: http://hdl.handle.net/10525/2396 ISBN: 978-954-8986-39-7 Appears in Collections: ADIS 2014

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